Biotechnology Test Preparation Set.2 comprising 100 MCQs that helpful in preparing Biotechnology Job Tests also include previously asked MCQs.
MCQs under the topic of Biotechnology covers all tests conducted by FPSC, NTS, KPSC, PPSC, SPSC, BPSC, OTS, UTS, UPS, PTS, CTS, ATS, ETEA or any other institution. Anyone who wants to pass job test comprising one paper or MCQs based test come on examtry.com and get prepared himself.
101 - Cosmid vectors are used for
cloning large fragments of DNA
102 - Cosmids lack
genes coding for viral proteins
103 - Cross protection against viruses in transgenic plants can be obtained by
inoculating the susceptible strain of a crop with a mild strain of a virus that helps in developing resistance against more virulent strain
104 - Crown gall tissue
both (a) and (b)
105 - Cryptic plasmids
do not exhibit any phenotypic trait
106 - Cultivated tomato species (Lycopersicon esculentum) has a high degree of resistance towards
L. peruviaanum
107 - Cultivated tomato species (Lycopersicon esculentum) is highly susceptible to
Meloidogyne spp. nematodes
108 - Cytoplast (somatic cell hybridization) is the process in which
both (a) and (b)
109 - Diethyl stilbestrol (DES), a synthetic estrogen causes
all of the above
110 - Difference between ? gt 10 and ? gt 11 vectors is that
? gt 11 is an expression vector
111 - Direct DNA uptake by protoplasts can be stimulated by
polyethylene glycol (PEG)
112 - Disaggregating of cells can be achieved by
all of the above
113 - DNA into fish is injected into
cytoplasm
114 - DNA is microinfected into the fertilized egg
before the fusion of male and female nuclei
115 - DNA microinjection into the egg has been used to produce which of the following transgenic animals?
All of these
116 - DNA replication is controlled at which of the following checkpoint?
G1
117 - During meiosis crossing over takes place at
pachytene stage
118 - During nitrogen fixation the enzyme nitrogenase catalyse the reaction. The reaction is high energy demanding which require approximately
18 ATP
119 - During recovery step of citric acid, calcium citrate formed is treated with which of the following acid to precipitate calcium?
H2SO4
120 - During sickle cell anemia which of the following takes place?
Glutamine change to valine
121 - During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
The cells do not have enough oxygen
122 - During which phase of growth of Penicillium chrysogenum maximum antibiotic production takes place
during the second phase
123 - During which phase, a cleavage furrow is formed in animal cells?
C
124 - Each chromosome contains two chromatids by the end of the __________ phase in the life cycle of a human cell.
S
125 - Electroporation is
the application of high voltage pulses
126 - EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
15-25 kb
127 - Embryo transfer (ET) refers to the technique by which fertilized ova are collected from the reproductive tract of a genetically
superior female and transferred to the inferior
128 - Encapsulation is necessary to produce and protect synthetic seeds. The encapsulation is carried out by various types of hydrogels, which are
soluble in water
129 - Enucleation of the cells can be achieved by treating the cells with
cytochalasin B
130 - Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
restriction endonucleases
131 - Establishment of embryogenic cell suspensions with high regeneration potential is a pre-requisite for successful
protoplast culture
132 - Ethanol is oxidized to acetic acid aerobically using
both (a) and (b)
133 - Ethanol is produced by
both (a) and (b)
134 - Excess CO2 suppress cell growth and productivity by
both (a) and (b)
135 - Exchange of segments between non homologous chromosomes is best known as
translocation
136 - Expression of antisense RNA in transgenic plants is a general method used to
reduce or eliminate the expression of individual genes
137 - Fermentation medium for oxytetracyclin (terramycin) consist of
CSL, starch, (NH4)2 SO4, sodium chloride and CaCO3
138 - Final alcohol content in wine varies from
both (a) and (b)
139 - Fining a wine is defined as
adding one substance to remove another
140 - fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is
Ura 3
141 - Foot and mouth disease (FMD) of animals is caused by
RNA virus
142 - For culturing, plasma from the adult chicken is preferred to mammalian plasma because
it forms a clear and solid coagulum even after dilution
143 - For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used?
All of the above
144 - For gene probes to be useful they must
both (a) and (b)
145 - For increased level of mannitol in transgenic tobacco plants which of the following gene is transferred?
Mannitol dehydrogenase
146 - For the rapid genetic progress, embryo transfer (ET)
will not be as potent a tool as AI (artificial insemination)
147 - For the recovery of citric acid after fermentation, Ca(OH)2 is added to the slurry to
precipitate calcium citrate
148 - For the transfer of whole individual chromosomes, they are isolated from the cells at
metaphase
149 - Fusion of karyoplast with the enucleated cell is achieved in presence of
polyethylene glycol
150 - Gene banks conserve stocks of
seed and vegetative material both
151 - Genetic change can occur only in
four ways
152 - Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?
AUUAAGGC
153 - Grist is
coarse powder obtained after milling of malted barley
154 - Gypsum is beneficial for
alkaline soil
155 - Hairy wing character of Drosophila is due to
duplication in region of chromosome
156 - Helper T cells assist in the functions of
certain B cells and other T cells
157 - High ethanol concentration
inhibits yeast growth
158 - High yield of chlorotetracyclin requires
continuous aeration
159 - Higher dissolved oxygen concentration in the culture media are toxic and lead to
all of the above
160 - How does CO2 help in the cell metabolism during cell culture?
It participates in the de novo synthesis of purines and pyrimidines
161 - How does dosage compensation in Drosophila occur?
Hypertranscription of male X chromosome
162 - How is air supply to the fermentor sterilized?
All of the above
163 - How is human insulin synthesized using recombinant DNA technology?
By using chemically synthesized DNA sequences for the two chains separately
164 - How long does it take to complete fermentation process in case of red wine?
3-5 days
165 - How long does it take to complete fermentation process in case of white wine?
7-11 days
166 - How many chromatids are there in a duplicated chromosome?
Two
167 - How many chromatids does a diploid body cell contain just prior to cell division?
92
168 - How many chromosomes are there in Caernorhabditis elegans?
6
169 - How many chromosomes are there in the budding yeast (S. cerevisiae)?
16
170 - How many nucleotides are there in Ss RNA molecule of picorna virus causing FMD?
8000
171 - Human growth hormone (hGH) is secreted by
pitutary gland
172 - Human insulin formed by recombinant DNA technology is known as
humulin
173 - Human serum albumin has been synthesized by
transgenic potato and tobacco plants
174 - Human serum albumin in case of transgenic plant is secreted in
transgenic leaf tissues
175 - Hypoxanthine can be measured by
hypoxanthine sensor
176 - If a winemaker wanted to make a deeply colored red wine, which of the following should not be done?
Use whole clusters
177 - If complex III were incorporated into an artificial lipid vesicle in order to demonstrate it's proton translocating ability in isolation, which of the following would be an appropriate electron donor?
Ubiquinol (UQH2)
178 - If starch containing substrates are used for ethanol production, yeast strain can't be used directly because
it doesn't contain amylases to hydrolyze starch
179 - If the ends of the following polysaccharide are pulled, which one would stretch the most?
Starch
180 - If the ester bonds of a triacylglycerol molecule's are broken then the kind of molecule, which would be soluble in water, is
glycerol
181 - If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective?
Mutant selection
182 - If the malolactic fermentation is not carried out,
the wine may be microbially unstable
183 - If the RBC of human were kept in 0.9% NaCl solution, what sort of change will occur to RBC?
No change to RBC
184 - If two genes show 50% recombination
both (a) and (b)
185 - In a __________ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area.
bioremediation
186 - In a callus culture
increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
187 - In a dihybrid cross, the progeny of an Fl individual (hetero2ygous for both characters) will show a phenotypic ratio of
1:1:1:1
188 - In a hot climate, grapes lose acidity because
of break down of malic acid
189 - In a plant tumour cell
only a small specific segment of callus T DNA is incorporated
190 - In an organism, the chromosome number is maintained constant because of
DNA duplication
191 - In angiosperm, the endosperm is
triploid
192 - In animal cell culture, particularly mammalian cell culture, transformation means
phenotypic modifications of cells in culture
193 - In case of Baculoviruses, the foreign gene
is inserted between the polyhedrin promoter and downstream sequences
194 - In case of incomplete dominance, the phenotypic ratio of a monohybrid cross will be
0.04306712963
195 - In genetic engineering, a chimera is
a plasmid that contains foreign DNA
196 - In human B cells and T cells are matured in the
bone marrow and thymus respectively
197 - In immuno-inflammatory diseases such as hemolytic anaemia, eczema etc.,
T8 cells are greatly reduced
198 - In India first genetically engineered vaccine against HBV was developed by
Shantha Biotechnics Pvt Ltd
199 - In koji process for the fermentation to produce citric acid,
A. niger is used with solid substrate as sweet potato
200 - In liquid surface culture process for the fermentation to produce citric acid,
A. niger floats on the surface of solution
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